Table 2.

Proportion of cells and organoids in different initial size bins one day after plating in each culture method

	% of organoids in size range (mean ± SD)			% of cells in size range (mean ± SD)
	Whole organoids	Partly dissociated	Suspension (150 k/mL)	Whole organoids	Partly dissociated	Suspension (150 k/mL)
<10 cells	7.0 ± 4.4	58.3 ± 9.3	70.6 ± 12.4	0.0026 ± 0.0023	0.308 ± 0.013	13.26 ± 7.77
10–1000 cells	34.3 ± 1.4	33.5 ± 11.7	29.4 ± 12.4	1.13 ± 0.068	9.14 ± 4.59	86.74 ± 7.77
>1000 cells	58.7 ± 3.0	8.20 ± 2.43	0 ± 0	98.7 ± 0.07	90.55 ± 4.60	0 ± 0

Day 1 organoid area measurements were taken from organoids cultured using the 3 methods outlined in Fig. 2a, then converted into approximate cell number as in Fig. 1f. n = 2 biological replicates, n (# of area measurements) = 120 whole embedded, 423 partly dissociated then embedded, 487 suspension culture. Units (single cells, small clusters, or large clusters) were then binned into sub-optimal (<10 or > 1000 cell) and optimal (100–1000 cell) sizes. The percentage by cell number (calculated from predicted organoid volume) and by number of measured units (cells or clusters) in each bin are presented