Table 3.

Estimated cost to expand 1 million MMTV-PyMT cells to > 150 million cells as organoids

	Traditional 3D culture (embedded in 100% Matrigel)			Suspension with 2% Matrigel culture
	Item	Quantity	Cost	Item	Quantity	Cost
	24 well plate	19 plates	$57.93	6 well plate, non-adherent	1 plate	$15.04
	GFR Matrigel	42.24 mL	$1314.17	T175 flask, agarose coated	1 flask	$10.95
	Organoid media	422 mL	$257.86	Organoid media +2% Matrigel	84 mL	$103.04
Total cost			$1629.96			$129.03
Hours of labor			≥ 12 h			≤ 3 h
# of days to reach target			16			8

Approximate cost (as of June 2020) of expanding 1 million cells to 167 million cells (i.e. the number of cells needed for a genome-wide CRISPR screen) either by plating in 100 μL Matrigel droplets in 24 well plates and lightly dissociating organoids after removal from gel to passage (traditional method) or by plating in suspension supplemented with 2% Matrigel and passaging to 150,000 viable cells/mL