Table 5.
Bioactivity of Tunisian okra pods (Abelmochus esculentus L. Moench).
| Abelmoschus esculentus L. Moench | |||
|---|---|---|---|
| Antioxidant activity; EC50 = mg/mL A | |||
| DPPH scavenging activity | 1.03 ± 0.02 | ||
| FRAP scavenging activity | 0.89 ± 0.03 | ||
| β-carotene bleaching inhibition | 0.47 ± 0.02 | ||
| Cytotoxic activity; IC50 = mg/mL B | |||
| NCl-H460 | 49.62 ± 0.02 | ||
| MCF-7 | 56.40 ± 0.30 | ||
| HeLa | 67.30 ± 0.50 | ||
| HepG-2 | 168.00 ± 7.00 | ||
| Anti-hyperglycaemic activity; IC50 = mg/mL C | |||
| α-amylase inhibition | 0.125 ± 0.02 | ||
| α-glucosidase inhibition | 0.110 ± 0.01 | ||
| Analgesic Activity | |||
| Groups | Concentration (mg/kg) | Number of Writhes | Inhibition of Writhing (%) |
| Control | ----- | 81.20 ± 0.40 | ------ |
| Tunisian okra pods | 10 | 59.70 ± 0.60 ** | 26.48 |
| 50 | 47.00 ± 0.40 ** | 42.09 | |
| 100 | 34.00 ± 0.30 *** | 58.11 | |
| Reference drug (ASL) | 200 | 30.00 ± 1.00 *** | 63.65 |
Values are expressed as mean ± S.E.M. (n = 6); ASL: Acetylsalicylate of lysine. A Trolox EC50 values: 0.030 ± 0.001 mg/mL (DDPH), 0.072 ± 0.002 mg/mL (FRAP), and 0.010 ± 0.001 mg/mL (β-carotene bleaching inhibition); B Ellipticine GI50 value: 0.91 ± 0.04 μg/mL (MCF-7), 1.03 ± 0.09 μg/mL (NCI-H460), 1.91 ± 0.06 μg/mL (HeLa), 1.1 ± 0.2 μg/mL (HepG2). C Acarbose IC50 values: 0.093 ± 0.001 mg/mL (α-amylase inhibition) and 0.28 ± 0.01 mg/mL (α-glucosidase inhibition). ** p ≤ 0.01 significant from control; *** p ≤ 0.001 significant from control.