Table 3.
Use of Box–Behnken design to investigate the optimization conditions for producing dextranase enzyme (unit/g) from P. roquefortii TISTR 3511.
| Condition | Significant Factor | Actual Value * | Predicted Value | ||
|---|---|---|---|---|---|
| Dextran (g/L) | Yeast Extract (g/L) | Inoculum Age (Days) | Dextranase Activity (unit/g.) | Dextranase Activity (unit/g.) | |
| 1 | 50 | 30 | 7 | 29.57 | 29.31 |
| 2 | 20 | 30 | 5 | 25.87 | 26.16 |
| 3 | 50 | 20 | 5 | 28.46 | 28.26 |
| 4 | 50 | 10 | 7 | 25.67 | 25.34 |
| 5 | 50 | 20 | 5 | 28.45 | 28.26 |
| 6 | 50 | 30 | 3 | 27.68 | 28.01 |
| 7 | 80 | 10 | 5 | 25.46 | 25.17 |
| 8 | 80 | 20 | 7 | 28.12 | 28.74 |
| 9 | 80 | 20 | 3 | 26.35 | 26.38 |
| 10 | 20 | 20 | 3 | 24.87 | 24.25 |
| 11 | 50 | 20 | 5 | 27.47 | 28.26 |
| 12 | 50 | 20 | 5 | 28.46 | 28.26 |
| 13 | 50 | 10 | 3 | 23.57 | 23.83 |
| 14 | 80 | 30 | 5 | 30.24 | 29.88 |
| 15 | 20 | 10 | 5 | 22.35 | 22.71 |
| 16 | 20 | 20 | 7 | 24.78 | 24.71 |
| 17 | 50 | 20 | 5 | 28.47 | 28.26 |
* Dextranase activity was determined under its suitable condition at pH 6 and 37 °C.