Table 1.
Ingredients and nutrient composition of experimental diets, g/kg as-fed basis.
| Ingredients, g/kg | Control | COS (1 g/kg) |
|---|---|---|
| Corn | 538.1 | 528.1 |
| Soybean meal (48.5% CP) | 345.2 | 345.2 |
| Soybean oil | 45.0 | 45.0 |
| Monocalcium phosphate1 | 17.0 | 17.0 |
| Limestone (38% Ca) | 15.5 | 15.5 |
| Salt | 4.0 | 4.0 |
| Vitamin–mineral premix2 | 3.0 | 3.0 |
| DL-Methionine | 3.5 | 3.5 |
| L-Lysine.HCl | 3.0 | 3.0 |
| Threonine | 0.7 | 0.7 |
| Chromic oxide premix3 | 25 | 25 |
| COS premix4 | 0 | 10 |
| DEX premix5,6 | 0 | 0 |
| Total | 1,000 | 1,000 |
| Calculated nutrients and energy content | ||
| CP, g/kg | 220 | 220 |
| ME, kcal/kg | 3,115 | 3,112 |
| Ca, g/kg | 9.7 | 9.7 |
| P, g/kg | 7.3 | 7.3 |
| Non-phytate P, g/kg | 4.8 | 4.8 |
| Ca: Total phosphorus | 1.3 | 1.3 |
Contained 16% Ca, 21% P.
Supplied the following per kilogram of diet: vitamin A, 5,484 IU; vitamin D3, 2,643 IU; vitamin E, 11 IU; menadione sodium bisulfite, 4.38 mg; riboflavin, 5.49 mg; D-pantothenic acid, 11 mg; niacin, 44.1 mg; choline chloride, 771 mg; vitamin B12, 13.2 μg; biotin, 55.2 μg; thiamine mononitrate, 2.2 mg; folic acid, 990 μg; pyridoxine hydrochloride, 3.3 mg; I, 1.11 mg; Mn, 66.06 mg; Cu, 4.44 mg; Fe, 44.1 mg; Zn, 44.1 mg; Se, 300 μg.
Prepared as 1 g of chromic oxide added to 4 g of ground corn.
1 g of chitosan oligosaccharide (COS) (Qingdao Yunzhou Biochemistry Co. Ltd., Jimo, Qingdao, China) added to 9 g of ground corn.
Prepared as 0.01 g of dexamethasone (DEX) (Alfa Aesar, Maryland) added to 9.99 g of ground corn.
Dexamethasone premix was added at the expense of corn to supply 0, 1 mg DEX/kg diet.