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The sequence of primers used for the qPCR analysis of selected microbial populations in cecal digesta samples at day 16.
| Target group or organism | Primer sequence (5′–3′) | Annealing T0 (C˚) | Reference |
|---|---|---|---|
| Bacillus spp. | F-GCAACGAGCGCAACCCTTGA | 63 | Zhang, et al. (2015) |
| R-TCATCCCCACCTTCCGGT | |||
| C. perfringens | F-ATGCAAGTCGAGCGAKG R-TATGCGGTATTAATCTYCCTTT Probe-5′-FAM-TCATCATTCAACCAAAGGAGCAATCC-TAMRA-3 |
60 | Rinttila, et al. (2004) |
