Table 7.

Effect of meat and bone meal, phytase, and antibiotics levels on bacterial quantification as log₁₀ genomic DNA copies per gram of cecal contents from broilers on day 16.

Effects	MBM	Phy	AB	Gizzard pH	Ileal pH	Cecal pH	Bacillus spp	C. perfringens
2-way interactions
Phy*AB
		500	−	2.87	5.87	6.41	8.34a	11.73
		500	+	2.73	5.76	6.21	7.82b	11.17
		1,500	−	2.83	5.69	6.40	7.55b	11.57
		1,500	+	2.88	5.69	6.24	8.23a	11.49
Main effects
MBM	−			2.78	5.60b	6.23b	8.16	11.21b
	+			2.87	5.91a	6.40a	7.81	11.77a
SEM				0.06	0.06	0.30	0.13	0.15
P > f
MBM				0.463	0.005	0.046	0.159	0.059
Phy				0.646	0.257	0.305	0.457	0.778
AB				0.707	0.607	0.067	0.757	0.275
MBM × Phy				0.234	0.051	0.072	0.464	0.100
MBM × AB				0.377	0.803	0.601	0.279	0.469
Phy × AB				0.403	0.585	0.431	0.021	0.406
MBM × Phy × AB				0.181	0.211	0.287	0.421	0.489

^a,bmeans in the same column within a main effect, 2-way interaction or treatment means with different superscripts are different (P < 0.05).

AB = Salinomycin 60 ppm in S, G, F; Zn bacitracin 100 ppm in S, G and 50 ppm in F.

Phy = Phytase (Quantum Blue 5G).

2-way or 3-way interaction separated by Tukey.

Abbreviations: AB, antibiotics; MBM, meat and bone meal.