Oil Red O staining showing effects of parental dietary linoleic acid on hepatic lipid accumulation in domestic pigeon squabs. Control, control group; LA1%, 1% linoleic acid addition group; LA2%, 2% linoleic acid addition group; LA4%, 4% linoleic acid addition group. (A) Oil Red O staining area was measured using Image-pro plus 6.0. Values are means ± SEM of 6 squabs. a,b Means sharing no common superscripts differ significantly (Tukey test, P < 0.05). (B) Representative Oil Red O staining of livers in the 4 experimental groups. Bar = 100 μm.