Table 2.
Primers used for quantitative real-time PCR analyses of gene expression in domestic pigeons.
| Genes | Primer | Sequence (5’→3′) | Length of the amplicon (bp) | Accession number1 |
|---|---|---|---|---|
| ACC | ACC-F | CTCATGGTCTTCGCCAACTGGA | 87 | XM_013367232.1 |
| ACC-R | CACGATGTAGGCACCGAACTT | |||
| FAS | FAS-F | AAACTGAAGGCTGCTGATAAGT | 184 | XM_005515764.1 |
| FAS-R | CCTCCAATAAGGTGCGGTGAT | |||
| CPT1 | CPT1a-F | TCGTCTTGCCATGACTGGTG | 143 | XM_013369225.1 |
| CPT1a-R | GCTGTGGTGTCTGACTCGTT | |||
| ACO | ACO-F | GGCATTGAGGAGTGTCGGA | 244 | XM_005503118.2 |
| ACO-R | GCACAGTCACAGATGGAGCA | |||
| PPARα | PPARα-F | AGAATAAGGAAGCCGAAGTTC | 104 | XM_021297326.1 |
| PPARα-R | GGAGAAGCCAGGGATAGATTTG | |||
| PPARγ | PPARγ-F | CCAGCGACATCGACCAGTT | 145 | XM_021288013.1 |
| PPARγ-R | GGTGATTTGTCTGTCGTCTTTCC | |||
| β-actin | β-actin-F | CCCATCTACGAAGGCTACGC | 150 | AB618546.1 |
| β-actin-R | CTTGATGTCACGCACAATTTC |
Abbreviations: ACC, acetyl-CoA carboxylase; ACO, acyl-CoA 1; CPT1, carnitine palmitoyltransferase 1; FAS, fatty acid synthase; PPARα, peroxisome proliferator-activated receptor α; PPARγ, peroxisome proliferator-activated receptor γ.
1
Accession number is from the National Center for Biotechnology Information (https://www.ncbi.nlm.nih.gov/).