Table 2.
Primers used for quantitative real-time PCR.
| Gene name1 | Accession number | Forward sequence (5′ to 3′) | Reverse sequence (5′ to 3′) |
|---|---|---|---|
| Beta-actin | NM_205518.1 | GAGAAATTGTGCGTGACATCA | CCTGAACCTCTCATTGCCA |
| Claudin-1 | NM_001013611 | TGGAGGATGACCAGGTGAAGA | GAGCCACTCTGTTGCCATA |
| ZO-1 | XM_413773.4 | CTTCAGGTGTTTCTCTTCCTCCTC | CTGTGGTTTCATGGCTGGATC |
| Occludin | NM_205128.1 | TCATCGCCTCCATCGTCTAC | TCTTACTGCGCGTCTTCTGG |
| TLR2 | NM_204278.1 | CCTGCAACGGTCATCTCAG | GTCTCAGGGCTTGTTCTTCAG |
| TLR4 | NM_001030693 | CTGACCTACCCATCGGACAC | GCCTGAGAGAGGTCAGGTTG |
| MyD88 | NM_001030962 | AGAAGGTGTCGGAGGATGGTG | GGGCTCCAAATGCTGACTGC |
| NF-κB p65 | NM_205129 | GTGTGAAGAAACGGGAACTG | GGCACGGTTGTCATAGATGG |
| IL-1β | NM_204524.1 | ACTGGGCATCAAGGGCTA | GGTAGAAGATGAAGCGGGTC |
| TNF-α | AY765397.1 | CTCCGCAGTACTCAGGACAGC | TCAGAGCATCAACGCAAAAGG |
1
ZO-1: zonula occludens-1; TLR2: toll-like receptor 2; TLR4: toll-like receptor 4; MyD88: myeloid differentiation factor 88; NF-κB P65: nuclear factor kappa B P65; IL-1β: interleukin 1 beta; TNF-α: tumor necrosis factor alpha.