Table 1.
The enriched sperm-related genes differentially expressed in the UVJ tissues of white Leghorn hens after insemination (day 9 vs. day 3 after AI).
| Genes | Potential function | Expression pattern | Log2 FC1 | Reference |
|---|---|---|---|---|
| AGO2 | Spermatogenesis-related gene | Seminiferous tubules in rat testis | −0.7723 | PMID:27029769 |
| ASB9 | Spermatogenesis-related gene | Mouse pachytene spermatocytes and spermatids | −0.6590 | PMID: 18776735 |
| CECR2 | Spermatogenesis-related gene | Mice testis | −0.7247 | PMID:22154806 |
| FGF13 | Spermatogenesis-related gene | Mouse testis | −2.6986 | PMID: 23554914 |
| LIPG | Spermatogenesis-related gene | Human testis | −0.8166 | PMID: 19780863 |
| GFRA1 | Related to spermatogenesis (subpopulations of spermatogonia) | Human testis | 1.2107 | PMID: 28827392 |
| HCK | Spermatogenesis-related gene | Bovine spermatozoa and testis | 0.6470 | PMID: 17926339 |
| NRG1 | Spermatogenesis-related gene | Nrg1(Ser−/−) mutant mice | 1.2480 | PMID: 18586089 |
| PIP5K1B | Spermatogenesis-related gene | Pip5k1a-knockout, Pip5k1b-Knockout, and Pip5k1a/Pip5k1b double knockout mice | 0.5860 | PMID: 22321832 |
| SMYD3 | Spermatogenesis-related gene | Human testis and spermatozoa | −1.5791 | PMID: 16081583 |
| MBOAT1 | Related to germline development | Drosophila germ cell | −0.5843 | PMID: 19864461 |
| CFTR | Regulate sperm capacitation | Human sperm | 0.68207 | PMID: 27714810 PMID:19923167 |
| CLIC5 | Regulate sperm function | Bovine epididymal spermatozoa | −1.1024 | PMID: 15147883 |
| DNAJB1 | Chaperone proteins regulating capacitation | 0.84846 | PMID: 17595329 | |
| ERBB4 | Related to sperm motility | Erbb4-knockout mice | −1.2368 | PMID:29453196 PMID:25058600 |
| LGALS3 | Regulate sperm function | Rat, bull testis and epididymis, and human semen prostasomes | 0.5908 | PMID: 19830550 PMID: 30241677 PMID:19830550 |
| PDE1A | Regulate sperm function | Human and mouse sperm | −0.7014 | PMID: 12135876 PMID: 15901640 |
| HES5 | Regulates sperm motility by epididymosomes | Mouse epididymis | −1.1295 | PMID: 26825631 |
| MLPH | sperm DNA methylation patterns | Human spermatozoal DNA | −0.6076 | PMID: 28566207 |
| HIP1 | Related to sperm counts and motility | HIP1 knockout mice | 0.8861 | PMID: 16967501 |
| SFRP1 | Regulates spermatid adhesion in the testis | Rats testis | −0.5969 | PMID: 23073828 |
| SLC22A18 | Responsible for the sperm phenotype | Human sperm | −0.5211 | PMID: 25762640 |
| SLC26A8 | Sperm tail differentiation and motility | Human and mouse male germ cells | 0.5117 | PMID: 17517695 PMID: 23582645 |
| FKBP6 | Related to male fertility | Equine testis and sperm | 1.49906 | PMID: 25495881 or 12764197 |
| GDF9 | Related to sperm quality | Holstein bulls and bovine Sertoli cells | −1.0501 | PMID: 23884762 PMID: 28332739 |
| HDAC4 | Related to male infertility (methylation and gene expression) | Human spermatozoal DNA | −0.5727 | PMID: 30358834 |
| KRT19 | Related to sperm maturation and male fertility | Rat efferent ducts | 0.6199 | PMID: 19906187 |
| RPL28 | Related to fertile and infertile | Human sperm | 0.5329 | PMID: 25973848 |
| CX3CL1 | Related to immune system | Human fallopian tube and ejaculated sperm | −0.7536 | PMID: 14747189 |
| EBP | Immune-related genes | Porcine endometrium | 0.5559 | PMID: 29155178 |
| GPX3 | Sperm antioxidant defences | Epididymal fluid in the goat | 0.6428 | PMID: 27677348 |
| NDRG1 | Sperm RNA elements | Human sperm | −0.5120 | PMID: 29199464 |
| PDGFRB | Associations with semen production traits | Chinese Holstein bulls | −0.7863 | PMID: 28673243 |
| SFMBT1 | Associate semen volume and total sperm number | Holstein-Friesian bulls | −0.6057 | PMID: 25465359 |
Abbreviations: AI, artificial insemination; UVJ, uterovaginal junction.
1
Log2 FC = Log2 foldchange of the gene expression changes day 9 vs. day 3 after AI.