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. 2020 Jan 24;99(2):708–718. doi: 10.1016/j.psj.2019.12.031

Table 5.

Seminal attributes in aged Ross 308 breeder roosters fed fish/corn oil with rosemary.

	Diets								P-value
	Basal diet	Fish oil (2%)	Corn oil (2%)	Fish/corn oil (50:50%)	Fish oil (2%)+ RLP	Corn oil (2%)+ RLP	Fish/corn oil (50:50%)+ RLP⁷	SEM pooled	Diet	Time	Diet × Time
Semen volume (mL)	0.36	0.44	0.34	0.45	0.47	0.43	0.50	0.09	NS	NS	NS
Concentratio (n)	3.58^b	3.66^b	3.64^b	3.76^b	4.40^a	3.41^b	4.67^a	0.05	<0.0001	<0.0001	<0.0001
Total motility%	80.92^b	81.04^b	80.28^b	80.61^b	86.31^a	79.85^b	86.77^a	0.06	<0.0001	<0.0001	<0.0001
Progressive motility%	40.29^b	39.47^b	39.11^b	39.32^b	46.51^a	37.90^b	45.83^a	0.55	<0.0001	<0.0001	<0.0001
VCL¹	162.10	164.04	162.37	166.40	165.50	160.60	165.85	1.96	NS	0.007	0.01
VSL²	68.26	70.75	66.77	67.32	68.20	68.40	68.67	0.67	0.05	0.0003	0.03
VAP³	40.27^a–c	38.18^b,c	40.66^a-c	40.67^a–c	44.67^a,b	37.34^c	46.37^a	0.93	NS	<0.0001	<0.0001
LIN⁶	42.18	43.16	41.31	40.52	41.32	45.14	41.50	1.05	NS	0.004	0.01
STR⁵	55.75^b,c	54.25^c	61.56^a–c	60.79^a–c	65.67^a,b	54.67^c	67.59^a	1.11	0.003	<0.0001	<0.0001
ALH⁴	7.81^b,c	7.25^c	7.65^b,c	8.90^b,c	10.01^a,b	8.76^b,c	11.42^a	0.33	0.007	<0.0001	<0.0001
Morphology%	16.48	16.76	15.97	15.85	15.54	16.38	13.27	0.50	NS	<0.0001	0.003
Membrane Integrity%	77.40^b,c	75.67^c	76.88^b,c	76.57^c	84.12^a	73.90^c	81.44^a,b	0.58	<0.0001	<0.0001	<0.0001
Viability%	80.67^a,b	76.17^b	77.59^a,b	78.94^a,b	80.62^a,b	76.98^b	82.50^a	0.58	NS	<0.0001	<0.0001
DNA Fr⁺	8.10	9.12	9.03	8.59	8.31	8.08	8.54	0.37	0.2	<0.0001	NS
DNA FR⁻	91.90	90.87	90.97	91.41	91.69	89.42	91.46	0.51	NS	<0.0001	<0.001
MDA (nmol/mL)	3.19^a,b	3.25^a	3.57^a	3.31^a	2.66^b,c	2.59^c	2.64^b,c	0.06	0.002	<0.0001	<0.0001

¹

VCL: average velocity measured over the actual point to point track followed by the cell.

²

VSL: average velocity measured in a straight line from the beginning to the end of track.

³

VAP: path velocity of the smoothed cell path.

⁴

LIN: average value of the ratio VSL/VCL; measured the departure of the cell track from a straight line.

⁵

STR: average value of the ratio VSL/VAP; measured the departure of the cell path from a straight line.

⁶

ALH: amplitude of lateral head displacement corresponding to the mean width of the head oscillation as the sperm swam.

⁷

Rosemary leaves powder.