Table 2.
Experimental diet composition and nutrient content (starter).
| Treatment |
||||||||
|---|---|---|---|---|---|---|---|---|
| Limestone particle size |
Fine |
Coarse |
||||||
| Ca and AvP level1 |
PC |
NC |
PC |
NC |
||||
| Phytase | 1,000 | 0 | 1,000 | 0 | 1,000 | 0 | 1,000 | 0 |
| Ingredient (%) | ||||||||
| Corn | 55.66 | 55.66 | 55.66 | 55.66 | 55.66 | 55.66 | 55.66 | 55.66 |
| Soybean meal 48% | 36.59 | 36.59 | 36.59 | 36.59 | 36.59 | 36.59 | 36.59 | 36.59 |
| Poultry by-product meal | 1.29 | 1.29 | 1.29 | 1.29 | 1.29 | 1.29 | 1.29 | 1.29 |
| Poultry fat | 2.50 | 2.50 | 2.50 | 2.50 | 2.50 | 2.50 | 2.50 | 2.50 |
| Deflouridated phosphorus | 1.55 | 1.55 | 0.68 | 0.68 | 1.55 | 1.55 | 0.68 | 0.68 |
| Limestone fine | 0.49 | 0.49 | 0.70 | 0.70 | 0.00 | 0.00 | 0.00 | 0.00 |
| Limestone coarse | 0.00 | 0.00 | 0.00 | 0.00 | 0.49 | 0.49 | 0.70 | 0.70 |
| Sodium chloride | 0.50 | 0.50 | 0.50 | 0.50 | 0.50 | 0.50 | 0.50 | 0.50 |
| DL-Methionine | 0.23 | 0.23 | 0.23 | 0.23 | 0.23 | 0.23 | 0.23 | 0.23 |
| Choline chloride, 60% | 0.20 | 0.20 | 0.20 | 0.20 | 0.20 | 0.20 | 0.20 | 0.20 |
| Mineral premix | 0.20 | 0.20 | 0.20 | 0.20 | 0.20 | 0.20 | 0.20 | 0.20 |
| Vitamin premix2 | 0.05 | 0.05 | 0.05 | 0.05 | 0.05 | 0.05 | 0.05 | 0.05 |
| L-lysine | 0.09 | 0.09 | 0.09 | 0.09 | 0.09 | 0.09 | 0.09 | 0.09 |
| Selenium premix3 | 0.05 | 0.05 | 0.05 | 0.05 | 0.05 | 0.05 | 0.05 | 0.05 |
| Coban4 | 0.05 | 0.05 | 0.05 | 0.05 | 0.05 | 0.05 | 0.05 | 0.05 |
| L-Threonine | 0.01 | 0.01 | 0.01 | 0.01 | 0.01 | 0.01 | 0.01 | 0.01 |
| Titanium | 0.50 | 0.50 | 0.50 | 0.50 | 0.50 | 0.50 | 0.50 | 0.50 |
| Ronozyme HiPhos®5 | 0.04 | 0.00 | 0.04 | 0.00 | 0.04 | 0.00 | 0.04 | 0.00 |
| Sand | 0.00 | 0.04 | 0.66 | 0.70 | 0.00 | 0.04 | 0.66 | 0.70 |
| Calculated nutrient content (%) | ||||||||
| Metabolizable energy (kcal/kg) | 2,958 | 2,958 | 2,958 | 2,958 | 2,958 | 2,958 | 2,958 | 2,958 |
| Crude protein | 22.50 | 22.50 | 22.50 | 22.50 | 22.50 | 22.50 | 22.50 | 22.50 |
| Calcium | 0.90 | 0.90 | 0.72 | 0.72 | 0.90 | 0.90 | 0.72 | 0.72 |
| Total phosphorus | 0.67 | 0.67 | 0.52 | 0.52 | 0.67 | 0.67 | 0.52 | 0.52 |
| Available phosphorus | 0.45 | 0.45 | 0.30 | 0.30 | 0.45 | 0.45 | 0.30 | 0.30 |
| Total lysine | 1.30 | 1.30 | 1.30 | 1.30 | 1.30 | 1.30 | 1.30 | 1.30 |
| Total methionine | 0.58 | 0.58 | 0.58 | 0.58 | 0.58 | 0.58 | 0.58 | 0.58 |
| Analyzed nutrient content (%), as fed | ||||||||
| Crude fat | 4.01 | 3.99 | 3.87 | 3.95 | 3.91 | 4.29 | 4.10 | 3.86 |
| Crude protein | 21.24 | 21.34 | 21.68 | 20.91 | 22.31 | 21.13 | 21.89 | 21.18 |
| Crude fiber | 3.30 | 3.40 | 3.60 | 3.40 | 3.50 | 3.30 | 3.40 | 3.40 |
| Ash | 5.13 | 4.84 | 4.62 | 4.89 | 5.44 | 5.59 | 5.27 | 5.12 |
| Calcium | 0.77 | 0.80 | 0.65 | 0.59 | 0.86 | 0.95 | 0.79 | 0.67 |
| Total phosphorus | 0.63 | 0.62 | 0.52 | 0.49 | 0.66 | 0.69 | 0.58 | 0.52 |
1
The Ca and available phosphorus (AvP) levels used in this design are referred to as positive control (PC, 0.9:0.45) and negative control (NC, 0.72:0.3).
2
Vitamin premix supplied the following per kg of diet: 6,614 IU vitamin A, 1,984 IU vitamin D3, 33 IU vitamin E, 0.02 mg vitamin B12, 0.13 mg biotin, 1.98 mg menadione (K3), 1.98 mg thiamine, 6.6 mg riboflavin, 11 mg d-pantothenic acid, 3.97 mg vitamin B6, 55 mg niacin, and 1.1 mg folic acid.
3
Selenium premix provided 0.2 mg Se (as Na2SeO3).
4
Coban supplied monensin sodium at 90 mg/kg of feed.
5
Phytase (RONOZYME HiPhos) was expected to provide 1,000 FYT/kg feed and was assigned a matrix value of 0.18% for Ca and 0.15% for AvP.