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Primer sets for the loop-mediated isothermal amplification assay.
| Primer name | Sequence 5′-3′ |
|---|---|
| DHBV-F3 | TGCAATGCGY1TTTCCAAGA |
| DHBV-B3 | GAGAAAAGGGCTGAGACCG |
| DHBV-FIP | CARAGAAATCCTGGGCATCCCCGAATTC2CTGGAGCCCAAAYCTCWCC |
| DHBV-BIP | TGCTAGTAGCAGCAGGCTYGC-ACKCCCATTGGAGCTTTCCT |
Italic text indicates degenerate oligonucleotides, Y = C + T, R = G + A, W = A + T, K = G + T
Bold text represents the EcoR I restriction site added between the F1c and F2).
