Table 4.
Effect of heat stress and glutamine on redox state and antioxidants of the thigh muscle in broilers.
| Item | Treatments1 |
SEM | P-value | ||||
|---|---|---|---|---|---|---|---|
| CON | HS | HS-0.5% Gln | HS-1.0% Gln | HS-1.5% Gln | |||
| MDA (nmol/mg protein) | 0.40a | 0.66d | 0.58c | 0.52b,c | 0.46a,b | 0.020 | <0.001 |
| GSH (mg/g protein) | 5.92a | 3.33b | 4.89a,b | 5.87a | 5.86a | 0.312 | 0.023 |
| SOD (U/mg protein) | 109.97a | 85.53c | 89.33b,c | 96.53a,b,c | 106.57a,b | 2.930 | 0.021 |
| GSH-Px (U/mg protein) | 25.88a | 19.36b | 23.08a,b | 25.17a | 26.91a | 0.734 | 0.003 |
| CAT (U/mg protein) | 3.80a | 2.29b | 2.79a,b | 3.17a,b | 3.85a | 0.179 | 0.015 |
| T-AOC (U/mg protein) | 2.84a | 1.98b | 2.74a,b | 3.56a | 3.40a | 0.144 | 0.004 |
a–cValues without common superscripts in the same row differ significantly (P < 0.05); Duncan's test was used to compare statistical differences among the CON, HS, HS-0.5% Gln, HS-1.0% Gln, and HS-1.5% Gln groups.
Abbreviations: CAT, catalase; CON, control; GSH, glutathione; GSH-Px, glutathione peroxidase; HS, heat stress; MDA, malondialdehyde; SOD, superoxide dismutase; T-AOC, total antioxidant capacity.
CON = broilers were kept in the normal-temperature environment and fed a basal diet; HS = broilers were kept in the circular heat stress environment and fed a basal diet; HS-0.5% Gln, HS-1.0% Gln, and HS-1.5% Gln = broilers were kept in the circular heat stress environment and fed basal diets supplemented with 0.5, 1.0, and 1.5% Gln.