Figure 3.
Micrographs showing the histology of the cecum in hens orally administered with dextran sodium sulphate (DSS group) or water (control group), and the effects of DSS on the localization of monocytes/macrophages in intestinal tissues. The cecum of hens in the control (A) and DSS (B) groups. Hematoxylin-Eosin (HE) staining. Leukocytes are distributed in the lamina propria in the cecum of hens in the DSS group (arrows). The luminal epithelial cells of the cecum in the DSS group had disintegrated or were lost (arrow head). Frozen sections of the cecum of hens in the control (C) and DSS (D) groups stained with an antimonocyte/macrophage antibody. Monocytes/macrophages are in the lamina propria (triangle arrows) in all sections (data not shown). E = mucosal epithelium; L = lumen; LP = lamina propria. Scale bars = 50 μm. (E) Frequencies of monocytes/macrophages in the lamina propria of all intestinal segments. Open bars are the control group, and orange filled bars are the DSS group. Values are the number of monocytes/macrophages in an area of 1 × 105 μm2 (n = 8). Asterisks (*, **) indicate significant differences between the control and DSS groups (P < 0.05 and P < 0.01, respectively).