Table 9.
Intestinal luminal concentrations of IL-10 and IFN-γ of 21-day-old broilers fed varying levels of sulfur amino acids in control conditions or challenged with Eimeria spp.
IL-10 (ng/mg protein) |
IFN-γ (pg/mg protein) |
||||||||
---|---|---|---|---|---|---|---|---|---|
Challenge1 | SID M+C (%)2 day 11–21 | Duodenum | Jejunum | Ileum | Cecum | Duodenum | Jejunum | Ileum | Cecum |
PBS | 0.6 | 116.25 | 251.40 | 54.62 | 31.13 | 3.79 | 9.56 | 1.95 | 2.17 |
0.8 | 150.60 | 120.40 | 52.84 | 51.28 | 3.71 | 5.09 | 1.85 | 1.36 | |
0.9 | 124.58 | 146.82 | 75.34 | 73.01 | 5.27 | 2.17 | 2.86 | 2.68 | |
1.0 | 166.96 | 155.02 | 84.78 | 27.24 | 4.08 | 3.16 | 2.73 | 1.58 | |
Eimeria | 0.6 | 230.03 | 159.54 | 64.36 | 67.73 | 13.29 | 20.82 | 2.46 | 9.07 |
0.8 | 258.64 | 208.50 | 42.28 | 49.70 | 18.46 | 24.23 | 1.59 | 1.46 | |
0.9 | 287.31 | 157.33 | 73.91 | 83.72 | 22.87 | 25.62 | 3.88 | 9.84 | |
1.0 | 317.40 | 148.25 | 55.99 | 96.49 | 15.37 | 20.49 | 2.35 | 10.66 | |
SEM | 17.39 | 18.04 | 4.61 | 9.57 | 1.52 | 2.72 | 0.45 | 1.42 | |
Main effect | |||||||||
0.6 | 173.14 | 205.47 | 59.49 | 49.43 | 8.54 | 15.19 | 2.21 | 5.62 | |
0.8 | 204.62 | 164.45 | 47.56 | 50.49 | 11.08 | 14.66 | 1.72 | 1.41 | |
0.9 | 205.95 | 152.08 | 74.63 | 78.37 | 14.07 | 13.90 | 3.37 | 6.26 | |
1.0 | 242.18 | 151.64 | 70.38 | 61.87 | 9.72 | 11.83 | 2.54 | 6.12 | |
PBS | 139.60b | 168.41 | 66.90 | 45.67 | 4.21b | 5.00b | 2.35 | 1.95b | |
Eimeria | 273.34a | 168.41 | 59.14 | 74.41 | 17.50a | 22.79a | 2.57 | 7.76a | |
P-value | |||||||||
SID M+C | 0.529 | 0.719 | 0.156 | 0.693 | 0.509 | 0.971 | 0.635 | 0.566 | |
Challenge | <0.001 | >0.999 | 0.400 | 0.142 | <0.001 | 0.001 | 0.811 | 0.044 | |
Interaction | 0.913 | 0.411 | 0.510 | 0.571 | 0.722 | 0.880 | 0.942 | 0.688 |
Means with different superscripts within a column were significantly different (P < 0.05).
On day 14, broilers were orally gavaged with either PBS or the Advent Coccidiosis Vaccine (Lincoln, NE; 100× vaccine dose; consisting of a blend of live Eimeria acervulina, Eimeria maxima, and Eimeria tenella oocysts). IL-10, interleukin-10; IFN-γ, interferon-γ; PBS, phosphate-buffered saline.
SID M+C, standardized ileal digestible methionine + cysteine.