Table 2.
Summary of conditions applied for characterization of studied food industry by-products by HPLC.
| Extracts |
RL |
RH |
GS |
P |
HYT |
H |
|---|---|---|---|---|---|---|
| Solvent | MeOH | Water | MeOH | DMSO | DMSO | DMSO |
| Dilution (mg/ml) | 0.2–4 | 5 | 3 | 4 | 5 | 5 |
| Temperature (ºC) | 30 | 30 | 30 | 30 | 30 | 30 |
| Flow rate (ml/min) | 0.75 | 1 | 1 | 1 | 1 | 1 |
| Mobile phase | Acetonitrile (65%), water (35%), and phosphoric acid (0.2%) | Acetonitrile (65%), water (35%), and phosphoric acid (0.2%) | (A) Acetic acid/water (1:99) and (B) acetonitrile | (A) Acetic acid/water (0.1:99.5) and (B) methanol | (A) Acetic acid/water (2.5:97.5) and (B) acetonitrile | (A) Methanol and (B) water (50:50 v/v) |
| Linear gradient | - | - | From 96% (A) and 4% (B) to 90% (A) in 25 min; changing to 87% (A) in 5 min; changing to 50% (A), before re-equilibrating in 10 min | 15 min with 100% (A) was followed by a linear gradient from 100% (A) to 90% (A) and 10% (B) in 15 min; 10 min maintained before equilibration in 10 min to the initial composition | From 95% (A) and 5% (B) to 75% (A) and 25% (B) in 20 min; changing to 50% (A) and (B) in 20 min; changing to 20% (A) and 80% (B), and re-equilibrated in 10 min | - |
| Total time (min) | 25 | 25 | 45 | 50 | 60 | 25 |
| Wavelength (nm) | 230 | 340 | 280 | 280 | 280 | 280 |
Abbreviations: DMSO, dimethyl sulfoxide; GS, grape seed; H, Harpagophytum; HPLC, high-performance liquid chromatography; HYT, hydroxytyrosol; MeOH, methanol; P, pomegranate; RH, rosemary extract; RL, rosemary extract (Nutrox OS).