Table 5.
Effect of different concentrations of cysteamine, ergothioneine, and serine on the MOT, PMOT, VAP, VSL, and VCL of frozen-thawed semen.
| Factor | MOT (%) | PMOT (%) | VAP (μm/s) | VSL (μm/s) | VCL (μm/s) | |
|---|---|---|---|---|---|---|
| Control | 0 | 61.08 ± 4.78a,b | 23.92 ± 2.08 | 63.79 ± 3.38a,b | 47.76 ± 3.26c | 111.60 ± 2.92a |
| Cysteamine (mmol) | 0.001 | 57.50 ± 4.64c,d | 24.50 ± 3.10 | 61.04 ± 3.60b | 49.80 ± 4.78b | 104.12 ± 4.49b,c |
| 0.002 | 60.08 ± 5.94b | 25.00 ± 2.04 | 64.46 ± 2.26a,b | 49.18 ± 4.93b | 109.82 ± 4.79a,b | |
| 0.004 | 57.08 ± 3.67d | 24.16 ± 3.03 | 64.24 ± 4.92a,b | 46.54 ± 4.03c | 108.84 ± 3.86b | |
| Ergothioneine (mmol) | 0.01 | 56.83 ± 4.97d,e | 24.08 ± 3.08 | 61.82 ± 3.02b | 46.00 ± 3.20c | 105.74 ± 4.69b,c |
| 0.02 | 56.50 ± 4.64e | 25.16 ± 2.65 | 63.80 ± 3.35a,b | 49.30 ± 4.48b | 110.62 ± 5.79a,b | |
| 0.04 | 57.58 ± 5.61c,d | 24.66 ± 2.90 | 64.34 ± 4.35a,b | 49.01 ± 3.51b | 111.06 ± 5.23a | |
| Serine (mmol) | 2 | 58.00 ± 4.37c | 25.58 ± 4.50 | 56.72 ± 2.62c | 44.38 ± 3.36d | 103.35 ± 4.24c |
| 4 | 62.66 ± 5.33a | 25.85 ± 3.39 | 66.98 ± 3.58a | 52.56 ± 5.30a | 112.46 ± 5.90a | |
| 6 | 60.33 ± 4.33b | 26.55 ± 2.87 | 62.91 ± 3.94b | 48.25 ± 4.89b,c | 109.49 ± 5.83a,b | |
The results are expressed as means ± standard deviations. N = 12. Different letters (a–e) within columns indicate significant differences (P < 0.05).
Abbreviations: MOT (%), percentage of total motile sperm; PMOT (%), percentage of sperm with progressive motility; VAP (μm/s), percentage of velocity average path; VCL (μm/s), percentage of velocity curvilinear; VSL (μm/s), percentage of velocity straight line.