Table 6.
Effects of different concentrations of cysteamine, ergothioneine, and serine supplementation of diluent on membrane integrity, acrosome integrity, functional mitochondria, and malondialdehyde of frozen-thawed chicken sperm.
| Factor | Membrane integrity (%) | Acrosome integrity (%) | Functional mitochondria (%) | MDA (μm/mL/150 × 106 spz) | |
|---|---|---|---|---|---|
| Control | 0 | 40.49 ± 3.00 | 36.88 ± 3.17b,c | 49.81 ± 3.25c | 1.98 ± 0.14a |
| Cysteamine (mmol) | 0.001 | 42.34 ± 3.97 | 35.49 ± 4.11c,d,e | 49.73 ± 2.80d | 1.40 ± 0.17b |
| 0.002 | 42.81 ± 4.61 | 34.26 ± 4.17e | 49.53 ± 3.51c | 1.39 ± 0.28b | |
| 0.004 | 41.93 ± 5.16 | 34.08 ± 3.88e | 48.59 ± 2.45c,d | 1.39 ± 0.15b | |
| Ergothioneine (mmol) | 0.01 | 40.17 ± 3.99 | 36.33 ± 3.97b,c,d | 50.06 ± 2.03c | 1.31 ± 0.19b |
| 0.02 | 41.75 ± 4.09 | 35.01 ± 4.10d,e | 49.65 ± 3.66c | 1.29 ± 0.26b | |
| 0.04 | 43.02 ± 4.33 | 36.24 ± 4.07c,d | 49.94 ± 4.35c | 1.36 ± 0.21b | |
| Serine (mmol) | 2 | 41.34 ± 3.37 | 37.21 ± 3.38b,c | 49.89 ± 5.64c | 1.39 ± 0.13b |
| 4 | 43.34 ± 4.29 | 40.99 ± 4.21a | 56.32 ± 3.89a | 1.38 ± 0.22b | |
| 6 | 41.56 ± 5.07 | 38.04 ± 5.86b | 51.78 ± 4.40b | 1.35 ± 0.15b | |
The results are expressed as means ± standard deviations. N = 12. Different letters (a–e) within columns indicate significant differences (P < 0.05).
Abbreviation: MDA, malondialdehyde.