. 2019 Dec 31;99(2):725–733. doi: 10.1016/j.psj.2019.12.002

Table 1.

Quantitative real-time PCR oligonucleotide primer sequences.

Type	Target gene	Primer sequence (5′-3′)	PCR product size (Kb)
Reference	GAPDH	F-GGTGGTGCTAAGCGTGTTAT	264
Reference	GAPDH	R-ACCTCTGCCATCTCTCCACA	264
Proinflammatory	IL-1β	F-TGGGCATCAAGGGCTACA	244
	IL-1β	R-TCGGGTTGGTTGGTGATG	244
	IL-6	F-CAAGGTGACGGAGGAGGAC	254
	IL-6	R-TGGCGAGGAGGGATTTCT	254
Th1	IL-2	F-TCTGGGACCACTGTATGCTCT	256
	IL-2	R-ACACCAGTGGGAAACAGTATCA	256
	IFN-γ	F-AGCTGACGGTGGACCTATTATT	259
	IFN-γ	R-GGCTTTGCGCTGGATTC	259
Tight junction proteins	JAM2	F-AGCCTCAAATGGGATTGGATT	59
	JAM2	R-CATCAACTTGCATTCGCTTCA	59
	Occludin	F-GAGCCCAGACTACCAAAGCAA	68
	Occludin	R-GCTTGATGTGGAAGAGCTTGTTG	68

Abberviations: F, forward primer; R, reverse primer.