Table 2.
Sequences of primers for quantitative real-time PCR.
| Target gene1 | Primer sequences (5′→3′) | Gene Bank No. | Product size (bp) |
|---|---|---|---|
| β-Actin | F: CACCACAGCCGAGAGAGAAAT | L08165 | 135 |
| R: TGACCATCAGGGAGTTCATAGC | |||
| TNF-α | F: GAGCGTTGACTTGGCTGTC | NM_204267 | 129 |
| R: AAGCAACAACCAGCTATGCAC | |||
| TRAF-2 | F: CCAGAACACGAAAGCAAGTG | XM_015279623.1 | 112 |
| R: ATGAGGGAATGGCAGGAAG | |||
| TNFRSF1B | F: TGCCTACTCACAGCCAACTG | NM_204439.3 | 126 |
| R:AGATGCTGCTCCTCCTGTTC | |||
| NF-κBp65 | F: GTGTGAAGAAACGGGAACTG | NM_205129 | 125 |
| R: GGCACGGTTGTCATAGATGG | |||
| IκB-α | F:GGCAGATGTGAACAAGGTGA | NM_001001472.2 | 118 |
| R:TATCTGCAGGTCAGCTGTGG | |||
| IFN-γ | F:GCTCCCGATGAACGACTTGA | NM_205149 | 150 |
| R:TGTAAGATGCTGAAGAGTTCATTCG |
1
TNF-α = tumor necrosis factor alpha; TRAF-2 = TNF receptor associated factor-2; TNFRSF1B = TNF receptor superfamily member 1B; NF-κBp65 = nuclear factor kappa-B p65 subunit; IκB-α = NF-κB inhibitor-alpha; IFN-γ = interferon-γ.