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. 2020 Oct 9;21(20):7432. doi: 10.3390/ijms21207432

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effect of hyperglycaemia on translocator protein (TSPO) and voltage-dependent anion-selective channel 1 (VDAC1) expression in myocardial tissue. (A) On the left, representative images showing that, in the STZ group, an up-regulation of TSPO expression was observed in comparison with the NPD group. On the right, quantification of TSPO expression related to immunohistochemistry analysis. (B) Densitometric analysis of VDAC1 expression assessed by Western blot assay showing the enhancement of protein level in mitochondrial fraction under hyperglycemia (on the right, representative images of Western blot) The data are presented as mean ± SEM. * p < 0.05; *** p < 0.001 vs. NPD; Mann–Whitney test, n = 6/group (immunohistochemistry analysis); t-test, n = 6/group (Western blot analysis).