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RP-HPLC profiles of (a) Acm2-precursor peptide and the reaction solutions after I2-oxidation in (b) 50% AcOH, (c) 50% MeOH/0.1 M HCl, (d) 50% i-PrOH/0.1 M HCl, and (e) 80% i-PrOH/0.1 M HCl with a linear gradient from 5 to 50% CH3CN in 45 min (l.0%/min). The asterisks (*) are the position of the expected retention time of the native type STh(6–18).
