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. 2020 Oct 9;21(20):7437. doi: 10.3390/ijms21207437

Figure 3.

Figure 3

The effect of inhibition of SRC kinases on key signaling factors. (A) WB analysis of SRC kinases and pY419–SRC protein expression (top) or FAK and pY925–FAK (bottom) in control-, dasatinib (DAS)- or PP2-treated Panc185 or Panc253 PaCSCs. The indicated ratios for pY419–SRC/SRC kinases and pY925–FAK/FAK were determined, and the fold-changes are shown, setting diluent (C)-treated cells as 1.0. (B) WB analysis of p27Kip1, cyclin D1, MYC, ERK1-2, pERK1-2, AKT, or pS473–AKT protein levels in control-, DAS-, or PP2-treated Panc185 or Panc253 PaCSCs. The indicated ratios for p27Kip1/ß-actin, cyclin D1/ß-actin, MYC/ß-actin, pERK1-2/ERK1-2, and pS473–AKT/AKT were determined and the fold-changes are shown, setting diluent (C)-treated cells as 1.0. Densitometric analysis of the indicated bands was determined using ImageJ software, and all values were normalized to ß-actin values, which was included as a loading and normalization control.