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. 2020 Oct 20;25(20):4815. doi: 10.3390/molecules25204815

Figure 2.

Figure 2

Rb3 attenuates expression of IL-6, IL-8 and IL-1β in response to P. gingivalis lipopolysaccharide (LPS). (A) Effect of Rb3 on HPLCs viability was tested using CCK-8 assay. Data were presented as means ± SE (n = 6). * p < 0.05 compared with normal control group. (B) Relative levels of IL-8 mRNA (24 h) determined by qPCR. (C) Relative levels of IL-6, IL-8 (24 h) and IL-1β (4 days) mRNA determined by qPCR. (D) Proteins levels of IL-6, IL-8 (24 h) and IL-1β (4 days) in supernatants were measured using enzyme-linked immunosorbent (ELISA). (B–D) Data were presented as means ± SE (n = 3), * p < 0.05 compared with LPS stimulation only group. SE, standard error.