Fig 8. Single nucleotide variant (SNV) analysis in B. glabrata FREPs between SUS and RES with RNA-Seq sequences.

SNV analysis of (A) FREP2 (Bg28617-PA); (B) FREP4 (Bg125-PA); (C) FREP3.1 (Bg96-PA); and (D) FREP3.2 (Bg204-PB). “Ref_BB02”, “SUS”, and “RES” are protein sequences of corresponding FREPs; in which RES and SUS are regenerated based on SNVs in their transcriptome data with BB02 as reference. Four featured sequences of “Signal peptide”, “IgSF1”, “IgSF2” and “FBG” are highlighted in colors. Each SNVs in RES from the two susceptible strains of BB02 and SUS are boxed in red. Any absolute matched sequences to identified peptides from FREP2, FREP3.2 of the plasma of SUS or RES from the pull-down experiments by the Li et al. [47] are framed in gray boxes with solid line. Sequences in FREP3.2 absolutely matched to the two RES-specific peptides from the pull-down experiments by Wu et al. [49] are framed in gray boxes with dash line.