Fig 3. Equivalent growth kinetics of ZIKV strains in mosquito and mammalian cells.

Mosquito (C6/36) and mammalian (Vero, A549) cells were infected with ZIKV strains at a multiplicity of infection of 0.01 PFU/cell in triplicate. Supernatant was harvested at each designated time point for titration by plaque assay. Overall kinetics and viral titers at individual timepoints were statistically equivalent (repeated measures ANOVA, p>0.05; Tukey’s multiple comparisons post-test, p>0.05).