Table 1. Hits from the screen selected for validation analyses.

Gene	Localization [16] and Function [43]	Role(s) in gene expression regulation
HRAS*	Plasma membrane and cytosol; GTPase, activation of signal transduction	Via Ras cascade activation (c-Raf, PI3K)
NUP93*	Nucleus; Nuclear pore assembly and maintenance	Smad4 nuclear translocation [27]; binds to active chromatin [26, 28, 29]
PKMYT1*	ER, Golgi; Tyrosine- and threonine-specific kinase; Golgi fragmentation, cell cycle regulation	Regulates β-catenin/Wnt signaling by phosphorylating and inactivating GSK3beta [31]
PRIM1*	Vesicles and nucleus; DNA primase	Involved in DNA replication
RUVBL1*	Nucleus; DNA-dependent ATPase and helicase	Chromatin remodeling; can drive oncogenesis as a co-factor for Myc and β-catenin [44, 45]
SF3A2*	Nucleus; Pre-mRNA splicing factor	Component of the spliceosome
TP53*	Nucleus; Tumor suppressor p53	Transcription factor; forms complex with CBP/CREB [46, 47]
ALG1	ER and nucleoli; biosynthesis of lipid-linked oligosaccharides	No known direct role(s)
MICAL3	Plasma membrane, cytosol and nucleus; actin filament disassembly, vesicle trafficking	No known direct role(s)

* = validated in follow-up analyses