Table 1. Differentially expressed proteins after both 24-hour and 30-hour exposure to 150 μM p,p‘-DDT identified in 2-DE experiments using three independent sets of samples.

Fold change—24 h	Fold change—30 h	Protein name	DTB No.	No. peptides	Coverage [%]	MS/MS confirmation	MW protein	pI
0.44	0.39	ECHM ↓ (enoyl-CoA hydratase, mitochondrial)	ECHM_HUMAN	5	25	ASGANFEYIIAEKR AQFAQPEILIGTIPGAGGTQR	31	8.3
10.83	8.57	GRP75 ↑ (75 kDa glucose-regulated protein)	GRP75_HUMAN	10	18	VQQTVQDLFGR NAVITVPAYFNDSQR STNGDTFLGGEDFDQALLR	74	5.9
16.10	- - -	GRP78* ↑ (78 kDa glucose-regulated protein)	GRP78_HUMAN	13	22	ITPSYVAFTPEGER VTHAVVTVPAYFNDAQR IEIESFYEGEDFSETLTR	72	5.1
2.40	2.42	GRP78** ↑ (78 kDa glucose-regulated protein)	GRP78_HUMAN	15	27	ITPSYVAFTPEGER IEIESFYEGEDFSETLTR	72	5.1
2.85	1.97	GRP78*** ↑ (78 kDa glucose-regulated protein)	GRP78_HUMAN	14	24	ITPSYVAFTPEGER VTHAVVTVPAYFNDAQR IEIESFYEGEDFSETLTR	72	5.1
0.48	0.50	HNRPF ↓ (heterogeneous nuclear ribonucleoprotein F)	HNRPF_HUMAN	6	17	HSGPNSADSANDGFVR ATENDIYNFFSPLNPVR	46	5.4
0.54	0.42	HSP27 ↓ (heat shock protein 27)	HSPB1_HUMAN	9	43	RVPFSLLR LFDQAFGLPR LATQSNEITIPVTFESR	23	6.0
0.41	0.43	IDH3A ↓ (isocitrate dehydrogenase [NAD] 3 subunit alpha, mitochondrial)	IDH3A_HUMAN	5	17	IAEFAFEYAR TIPIDGNFFTYTR	40	6.5
0.41	0.26	K2C8 ↓ (keratin, type II cytoskeletal 8)	K2C8_HUMAN	7	15	LEGLTDEINFLR ASLEAAIADAEQRGELAIK	54	5.5
0.51	0.36	VIME ↓ (vimentin)	VIME_HUMAN	16	43	ISLPLPNFSSLNLR EMEENFAVEAANYQDTIGR QVQSLTCEVDALKGTNESLER	54	5.1

The table covers the proteins that changed expression after both 24 hours and 30 hours of exposure. The table includes fold change after 24 hours and 30 hours, protein name, SwissProt database number, the number of peptides matched to the identified protein, sequence coverage, peptide sequences confirmed by MS/MS, theoretical molecular weight, and pI of the protein.

*, **, *** mark different spots identified as the same protein.