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Binding of FGF2 and FGF2 conjugates to a recombinant extracellular fragment of FGFR1-α-IIIc fused to Fc determined by biolayer interferometry. The biotinylated FGFR1c was immobilized on high-precision streptavidin biosensors (SAX), and then association and dissociation of FGF2 or FGF2 conjugates were monitored for 400 s. The red lines represent fitting curves according to a 1:1 model.
