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. 2020 Jun 5;17(7):2734–2748. doi: 10.1021/acs.molpharmaceut.0c00419

Figure 6.

Figure 6

Confocal microscopy imaging of endocytosed FGF2 and FGF2 conjugates. U2OS-R1 cells were transfected with Rab5-GFP (early endosome marker) or LAMP1-GFP (lysosome marker). Subsequently, the cells were incubated with 500 ng/mL of DyLight550-labeled FGF2 or conjugates on ice for 40 min and then shifted to 37 °C for 35 min in the case of Rab5-GFP-transfected cells or 50 min for LAMP1-GFP-transfected cells. Scale bars correspond to 10 μm. Insets: magnified views of boxed regions in the main images.