Table 2. Cytotoxicity of FGF2 Conjugates and Free AY in Different Cell Lines.
| cell
lines |
|||||
|---|---|---|---|---|---|
| U2OS-R1 | DMS114 | NCI-H520 | U2OS | HCC15 | |
| Preparation | EC50 [nM] | ||||
| FGF23xC-(tvAY)3 | 0.11b | 0.035 | 60.1 | nta | nt |
| FGF23xC-(PEG4-tvAY)3 | 0.18b | 0.033 | 50.5b | nt | nt |
| FGF23xC-(PEG27-tvAY)3 | 0.23 | 0.039 | 27.7b | nt | nt |
| FGF21xC-PEG27-(tvAY)3 | 0.95 | 0.019 | 158.0 | nt | nt |
| AY | nt | nt | nt | nt | nt |
a
nt, nontoxic in the studied concentration range. For statistical analysis between FGF21xC-PEG27-(tvAY)3 and other conjugates, a t test was applied using SigmaPlot software.
b
p < 0.05 was considered statistically significant; n = 3.