Figure 7.
Effect of aprepitant/itraconazole on the ROS levels in the presence (+) or absence (-) of iron. Carboxy-H2DCFDA probe was used to analyze ROS levels within C. auris AR0390 in the presence (+) or absence of iron source (FeSO4 50 µM). PBS suspensions of C. auris cells, at 1× 106 CFU/ml, were treated with either aprepitant (APR, 10 µg/ml), itraconazole (ITC, 1 µg/ml), or a combination of both for 3 h, and then were treated with H2DCFDA for 1 h, before measuring the fluorescence intensity as an indication of ROS levels. Data are shown as means ± SD. An asterisk (*) indicates statistical significance (P < 0.05) between treated and untreated groups (P < 0.05), whereas #, indicates statistical significance (P < 0.05) between treatment groups in the presence or absence of iron. Statistical significance was assessed by one-way ANOVA using Dunnett’s test for multiple comparisons