Figure 9.
In vivo efficacy of aprepitant/itraconazole in Caenorhabditis elegans infected with C. auris. C. elegans nematodes were infected with 1 × 107 CFU C. auris AR0390 and then treated with aprepitant (APR), at 10 µg/ml, and itraconazole (ITC), at 1 µg/ml, either alone or in combination. Untreated worms served as a negative control. (a) C. auris AR0390 CFU burden/worm 24 h post-treatment. An asterisk (*) indicates statistical significance (P < 0.05) relative to the untreated control, while # indicates statistical significance (P < 0.05) compared to the ITC treatment. Statistical significance was assessed by one-way ANOVA using Dunnett’s test for multiple comparisons. (b) Kaplan-Meier survival curve, assessed by log-rank test for significance, of C. elegans nematodes infected by C. auris AR0390 and treated with APR (10 µg/ml), ITC (1 µg/ml), or a combination of both agents