Figure 1.
Resveratrol inhibits the growth of subcutaneous Hepa1-6 tumors in C57BL/6 mice. Individual C57BL/6 mice were injected subcutaneously with 5 × 106 Hepa1-6 cells. One week after implantation, mice were treated with vehicle or resveratrol for 21 days. Body weight gains (a) and tumor volumes (b) were measured every three days during the treatment. The solid line represents the value of a single mouse, the long dotted line and the short dotted line represent the mean and SD, respectively, pooled from 4 mice. Images of tumors at the end of the treatment are also shown (c). Paraffin sections were prepared for immunohistochemical staining using anti-PCNA antibody (d), TUNEL staining (e) or immunofluorescence staining using anti-CD31 antibody (f), and images were taken using microscopy (200×) (n = 4 mice/group). Phosphorylated-STAT3 (p-STAT3) and total STAT3 expression levels in the tumor tissue derived from resveratrol- or vehicle-treated mice (n = 3 mice/group) were measured via Western blotting (g). Representative images from each experiment are shown. Data are expressed as mean ± SD from a representative of three independent experiments (M, mice; Res, resveratrol; ns, nonsignificant; two-tailed t-test: *p < 0.05, **p < 0.01 vs. Vehicle)