Figure 3.

Resveratrol treatment decreases the percentages of M2-type macrophages in Hepa1-6 tumor-bearing mice. (a) Single-cell suspensions isolated from tumor tissue of mice 21 days after treatment with resveratrol were prepared, stained with anti-CD45, F4/80, CD11b and CD206 antibodies and analyzed via FACS. (b) Immunofluorescence analysis (400×) of M2-type macrophages stained with anti-F4/80 and CD206 antibodies in the tumor tissue. (c) Peritoneal cavity washes (PCW) and spleen cells were isolated from the tumor-bearing mice and the frequencies of F4/80⁺ CD11b⁺ CD206⁺ M2-type macrophages were enumerated by FACS analysis. Data of column graphs are presented as mean ± SD (n = 4 mice/group; two-tailed t-test: *p < 0.05 and **p < 0.01 compared to Vehicle). One representative of three separate experiments is shown