Figure 4.

Resveratrol treatment increases IFN-γ-expressing CD8⁺ T cells in the Hepa1-6 tumor-bearing mice. Tumor-infiltrating lymphocytes, spleen and draining lymph node (LN) cells were isolated from tumor-bearing mice of resveratrol- or vehicle-treated group on day 21. Then 1 × 10⁶ cells were incubated in the presence of PMA/Ionomycin and monensin as described in the methods. The percentages of CD8⁺IFN-γ⁺ T cells from tumors, spleens or lymph nodes were enumerated by FACS analysis after intracellular staining of IFN-γ. Gating strategy for dot plots was indicated in supplemental materials. Data are shown as mean ± SD from a representative of three independent experiments (Res, resveratrol; n = 4–5 mice/group; two-tailed t-test: **p < 0.01 compared to Vehicle)