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. 2020 Oct 24;9(1):1829346. doi: 10.1080/2162402X.2020.1829346

Figure 4.

Figure 4.

Resveratrol treatment increases IFN-γ-expressing CD8+ T cells in the Hepa1-6 tumor-bearing mice. Tumor-infiltrating lymphocytes, spleen and draining lymph node (LN) cells were isolated from tumor-bearing mice of resveratrol- or vehicle-treated group on day 21. Then 1 × 106 cells were incubated in the presence of PMA/Ionomycin and monensin as described in the methods. The percentages of CD8+IFN-γ+ T cells from tumors, spleens or lymph nodes were enumerated by FACS analysis after intracellular staining of IFN-γ. Gating strategy for dot plots was indicated in supplemental materials. Data are shown as mean ± SD from a representative of three independent experiments (Res, resveratrol; n = 4–5 mice/group; two-tailed t-test: **p < 0.01 compared to Vehicle)