Figure 8.

Resveratrol inhibits the generation of CD8⁺CD122⁺ Tregs invitro.

(A) CD8⁺CD122⁻ T cells were FACS-sorted from the spleen of naïve mice and then cultured in the presence of anti-CD3/CD28 mAbs, IL-2 and different concentrations of resveratrol for 4 days. Cytotoxicity was performed using CCK-8 assays. (B and C) CD8⁺CD122⁻ T cells were cultured in the presence or absence of anti-CD3/CD28 mAbs, IL-2, IL-15 and/or resveratrol as indicated for 4 days. The frequencies of CD8⁺CD122⁺ Tregs were detected via FACS analysis. Representative dot plots (B) or quantification data (C) are also shown. Data are presented as mean ± SD (Two-tailed t-test: **p < .01). One representative of three separate experiments is shown.