Figure 3. Avoidance requires activation of BLA projections to VS.
(A) Schematic of ChR2 virus infusion and optic probe placement. (B) Percent time on platform during the last day of avoidance conditioning (Cond; No Laser), and 4 Hz and 15 Hz Laser tests. (C) Timecourse of avoidance during 4 Hz Laser revealed that BLA-VS ChR2 (n = 7) rats showed no significant difference in percent time on platform compared to eYFP (n = 7) controls (repeated-measures ANOVA, post-hoc Tukey). (D) Same conventions as in panel C during 15 Hz Laser. (E) 15 Hz photoactivation of VS-BLA projections reinstates avoidance following extinction (ChR2 n = 7, eYFP n = 7). (F) Schematic of ArchT virus infusion and optic probe placement. (G) Percent time on platform during the last day of avoidance conditioning (Cond; No Laser), and Test (Tone with Laser). (H) Timecourse of avoidance during Test revealed that ArchT (n = 7) rats showed impaired avoidance compared to eYFP (n = 14) controls (repeated-measures ANOVA, post-hoc Tukey). All data are shown as mean ± SEM. *p<0.05, **p<0.01.
Figure 3—figure supplement 1. Spread of AAV expression and location of optic probes.
