Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Recombinant DNA reagent | pGEX-TEV |
Choi et al., 2012
https://doi.org/10.1074/jbc.M511338200 |
Ampicillin resistance; expression in bacterial cultures; pGEX-KG plasmid (ATCC) with a new TEV protease site Contact Weis lab for distribution |
|
| Recombinant DNA reagent | pGEX-4T-3 | GE Healthcare | 28-9545-52 | Vector for thrombin-cleavable GST fusion protein expression in bacteria |
| Strain, strain background (Escherichia coli) | BL21 (DE3) Codon-Plus RIL | Agilent | 230245 | Strain for expressing recombinant proteins |
| Software, algorithm | RELION3 3.0.8 |
He and Scheres, 2017
https://doi.org/10.1016/j.jsb.2017.02.003 Scheres, 2012https://doi.org/10.1016/j.jmb.2011.11.010 Zivanov et al., 2018https://doi.org/10.7554/eLife.42166 |
RRID:SCR_016274 | |
| Software, algorithm | MotionCor2 1.3.0 |
Zheng et al., 2017
https://doi.org/10.1038/nmeth.4193 |
RRID:SCR_016499 | |
| Software, algorithm | Gctf 1.06 |
Zhang, 2016
https://doi.org/10.1016/j.jsb.2015.11.003 |
RRID:SCR_016500 | |
| Software, algorithm | CTFFIND4 4.1.5 | Rohou and Grigorieff, 2015https://doi.org/10.1016/j.jsb.2015.08.008 | RRID:SCR_016732 | |
| Software, algorithm | pyCoAn 0.3.0 | Volkmann and Hanein, 1999 https://doi.org/10.1006/jsbi.1998.4074 | Revision 1419 | |
| Software, algorithm | Phenix 1.17.1 | Afonine et al., 2018https://doi.org/10.1107/S2059798318006551 | RRID:SCR_014224 | |
| Software, algorithm | Coot 0.8.9 | Emsley et al., 2010https://doi.org/10.1107/S0907444910007493 | RRID:SCR_014222 | |
| Software, algorithm | EMRinger |
Barad et al., 2015
https://doi.org/10.1038/nmeth.3541 |
Via Phenix | |
| Software, algorithm | MolProbity |
Chen et al., 2010
https://doi.org/10.1107/S0907444909042073 |
RRID:SCR_014226
Via Phenix |
|
| Software, algorithm | ResMap 1.14 |
Kucukelbir et al., 2014
https://doi.org/10.1038/nmeth.2727 |
||
| Software, algorithm | SBGrid | Morin et al., 2013https://doi.org/10.7554/eLife.01456 | RRID:SCR_003511 | |
| Software, algorithm | UCSF Chimera 1.14 | Pettersen et al., 2004https://doi.org/10.1002/jcc.20084 | RRID:SCR_004097 | |
| Software, algorithm | GraphPad Prism 8.0.2 | GraphPad Software, Inc | Version 263 RRID:SCR_002798 |