Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Recombinant DNA Reagent | pET His-Strep-TEV and pET His-Strep-TEV-Halo vectors | This paper | Ampicillin resistance; expression vectors for E. coli
Contact Alushin Lab for distribution |
|
| Recombinant DNA Reagent | pFastBac Dual Expression Vector | ThermoFisher | 10712024 | Expression vectors for sf9 insect cells |
| Gene (H. sapiens) | Vinculin; HsVCL isoform 1 | Synthesized by Genewiz | UniProt:P18206-2 | |
| Gene (H. sapiens) | Metavinculin; HsVCL isoform 2 | Synthesized by Genewiz | UniProt:P18206-1 | |
| Gene (H. sapiens) | αE-catenin; HsCTNNA1 | Addgene # 24194 | UniProt:P35221 | |
| Strain, strain background (E. coli) | Rosetta2 (DE3) | Novagen | 71400–4 | E. coli strain for protein expression |
| Cell line (S. frugiperda) | Gibco Sf9 insect cell | ThermoFisher | 11496015 | Insect cell line for protein expression |
| Transfected construct (S. frugiperda) | Gibco Bac-to-Bac Baculovirus Expression System | ThermoFisher | 10359016 | Insect cell line transfection kit for protein expression |
| Chemical compound, drug | Halo-tag ligand amine O4 | Promega | P6741 | Synthetic precursor for the fluorescent dye JF-646-Halo |
| Chemical compound, drug | JF-646 NHS-ester building block | TOCRIS | 6148 | Synthetic precursor for the fluorescent dye JF-646-Halo |
| Chemical compound, drug | HaloTag Alexa Fluor 488 Ligand | Promega | G1001 | |
| Chemical compound, drug | Alexa Fluor 555-Phalloidin | ThermoFisher | A34055 | |
| Biological sample (O. cuniculus) |
Actin Protein (Rhodamine): Rabbit Skeletal Muscle | Cytoskeleton, Inc | AR05-C | |
| Biological sample (O. cuniculus) |
Actin Protein (Biotin): Rabbit Skeletal Muscle | Cytoskeleton, Inc | AB07-C | |
| Other | Streptavidin Coated Polystyrene Particles | Spherotech | SVP-40–5 | Used in optical trapping experiments |
| Other | C-flat Holey Carbon Grids for TEM - Gold Only | EMS | CF313-100-Au | Used in cryo-EM experiments |
| Software, algorithm | C-trap viewer | LUMICKS | http://www.nat.vu.nl/~iheller/download.html | |
| Software, algorithm | TWOM viewer | LUMICKS | http://www.nat.vu.nl/~iheller/download.html | |
| Software, algorithm | ImageJ 1.5 |
Schneider et al., 2012
https://doi.org/10.1038/nmeth.2089 |
RRID:SCR_003070 | ImageJ plugin for TIRF image analysis:https://github.com/alushinlab/ActinEnrichment |
| Software, algorithm | RELION 3.0 |
He and Scheres, 2017
https://doi.org/10.1016/j.jsb.2017.02.003 Schneider et al., 2012 https://doi.org/10.1016/j.jmb.2011.11.010 Zivanov et al., 2018 https://doi.org/10.7554/eLife.42166 |
RRID:SCR_016274 | |
| Software, algorithm | CTFFIND 4.1.5 |
Rohou and Grigorieff, 2015
https://doi.org/10.1016/j.jsb.2015.08.008 |
RRID:SCR_016732 | |
| Software, algorithm | Phenix 1.17.1 |
Afonine et al., 2018
https://doi.org/10.1107/S2059798318006551 |
RRID:SCR_014224 | |
| Software, algorithm | Coot 0.8.9 |
Emsley et al., 2010
https://doi.org/10.1107/S0907444910007493 |
RRID:SCR_014222 | |
| Software, algorithm | UCSF Chimera 1.14 |
Pettersen et al., 2004
https://doi.org/10.1002/jcc.20084 |
RRID:SCR_004097 | |
| Software, algorithm | UCSF ChimeraX 1.0 |
Goddard et al., 2018
https://doi.org/10.1002/pro.3235 |
RRID:SCR_015872 | |
| Software, algorithm | GraphPad Prism 8 | GraphPad Software, Inc | RRID:SCR_002798 |