Table 1. Cryo-EM data collection, refinement, and validation statistics.
| Metavinculin ABD‒F-actin (EMDB-20844, PDB 6UPW) |
α-catenin ABD‒F-actin (EMDB-20843, PDB 6UPV) |
|
|---|---|---|
| Data collection and processing | ||
| Microscope | Titan Krios | Titan Krios |
| Voltage (kV) | 300 | 300 |
| Detector | K2 Summit | K2 Summit |
| Magnification | 29,000 | 29,000 |
| Electron exposure (e‒/Å2) | 60 | 60 |
| Exposure rate (e–/pixel/ s) | 6 | 6 |
| Calibrated pixel size (Å) | 1.03 | 1.03 |
| Defocus range (µm) | ‒1.5 to ‒3.5 | ‒1.5 to ‒3.5 |
| Helical symmetry | C1 | C1 |
| 27.06 Å rise | 27.03 Å rise | |
| −167.07° twist | −166.88° twist | |
| Initial filament segments (no.) | 237,503 | 540,533 |
| Final filament segments (no.) | 215,369 | 414,486 |
| Map resolution (Å) | 2.94 | 3.24 |
| FSC threshold | 0.143 | 0.143 |
| Refinement | ||
| Initial model (PDB ID) | 3JBK, 3J8A | 4IGG, 3J8A |
| Model resolution (Å) | 2.98 | 3.28 |
| FSC threshold | 0.5 | 0.5 |
| Map sharpening B factor (Å2) | ‒58 | ‒85 |
| Model composition | 5 actin protomers, 2 actin-binding domains | |
| Non-hydrogen atoms | 16,896 | 17,233 |
| Protein residues | 2152 | 2201 |
| Ligands | 5 Mg.ADP | |
| B factors (Å2) | ||
| Protein | 53.41 | 71.59 |
| Ligand | 47.29 | 56.65 |
| R.M.S. deviations | ||
| Bond lengths (Å) | 0.012 | 0.012 |
| Bond angles (°) | 0.860 | 0.833 |
| Validation | ||
| MolProbity score | 1.23 | 1.33 |
| Clash score | 3.32 | 4.63 |
| Poor rotamers (%) | 0.06 | 0.05 |
| Ramachandran plot | ||
| Favored (%) | 97.46 | 97.56 |
| Allowed (%) | 2.54 | 2.44 |
| Disallowed (%) | 0.00 | 0.00 |
| EMRinger Score | 4.40 | 3.87 |