Fig. 5.

Flowcytometry assay to evaluate the effects of the MSCs, Ator and Ator + MSCs on the C6 cell apoptosis induction which compared with non-treated condition. a Histograms of harvested and stained C6 cells b Comparison of C6 cells death rates after 72 h treatment. Harvested C6 cells were stained with FITC-conjugated annexin V and propidium iodide and analyzed by the flowcytometry whereas annexin V and PI identify viable cells (annexin V−, PI−), early apoptotic cells (annexin V+, PI−), late apoptotic or necrotic cells (annexin V+, PI+) and necrotic cells (annexin V−, PI+). c Cell cycle distribution of C6 cells in different conditions including non-treated and treated by Ator, MSCs and combination of Ator with MSCs d Quantitative analysis of different cell phase populations. (Color figure online)