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. 2020 Sep 26;47(10):7607–7621. doi: 10.1007/s11033-020-05828-2

Table 1.

Band patterns that can be obtained using the universal, codominant CAPS marker for the detection of HOR3 and HOR4 type mutations in the BnaA.FAD2 gene of rapeseed

No HOR3 type mutation HOR4 type mutation DNA fragments (pattern)
1 Wild-type homozygote Wild-type homozygote 732 bp
2 Mutated homozygote Wild-type homozygote 410 bp, 322 bp
3 Heterozygote Wild-type homozygote 732 bp, 410 bp, 322 bp
4 Wild-type homozygote Mutated homozygote 488 bp, 244 bp
5 Wild-type homozygote Heterozygote 732 bp, 488 bp, 244 bp
6 Heterozygote1 Heterozygote1 488 bp, 410 bp, 322 bp, 244 bp

1HOR3 and HOR4 type mutations are in the repulsion phase

Profiles 1–5 were obtained experimentally and are shown in Fig. 3