Skip to main content
. 2020 Oct 26;11(10):920. doi: 10.1038/s41419-020-03119-z

Fig. 5. Functional CFTR can restore a more epithelial phenotype and confers resistance to TGF-β1-induced EMT.

Fig. 5

A Representative Western blots showing the effects of VX-445, VX-661 and/or VX-770 on CFTR, E-cadherin, N-cadherin, CK18 and vimentin in polarized CFBE cells. Calnexin was used as a loading control. Treatment with correctors VX-445 and VX-661 rescues F508del-CFTR increasing the amount of band C (arrowhead). B Quantification by densitometry of the protein expression detected by WB in A. Data is normalized to loading control and to negative control (DMSO) and showed as arbitrary units (A.U.), mean ± SEM. Asterisk indicates significant difference between negative control and treatment (unpaired t-test, p < 0.05). (n = 4). C Representative Western blots showing the effects of TGF-β1 and TGF-β1 with VX-445, VX-661 and VX-770 on CFTR, E-cadherin, N-cadherin, CK18 and vimentin in polarized CFBE cells. Calnexin was used as a loading control. D Quantification by densitometry of the protein expression detected by WB in C. Data are normalized to loading control and showed as arbitrary units (A.U.), mean ± SEM. Asterisk indicates significant difference between negative control and treatment (unpaired t-test, p < 0.05). (n = 3–4).