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. 2020 Oct 26;10:18290. doi: 10.1038/s41598-020-75222-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of the different anaerobic electron acceptors on the growth of Trichococcus pasteurii on TSA, TSA + vitamins, TSA + nitrate, TSA + sulfate, and TSA + Fe³⁺ at pH 7 under low-PTA conditions. Samples were taken at different incubation times up to 28 days. Cells were applied to the agar plates as multiple, 2.5-µL drops (~ 2.5 × 10⁵ cells/drop), squares of agar/cells were cut out, and subsequent MPN assays completed to estimate the numbers of viable cells over time. The data are reported as log10 (N/N₀) in which N₀ = viable cell numbers at T = 0 and N = viable cell numbers at a specific point between 3 and 28 days (n = 6; error bars are standard deviations).