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. 2020 Oct 26;10:18221. doi: 10.1038/s41598-020-75212-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Evaluation of gas exchange parameters from E. grandis seedlings co-inoculated with N33 and M25 and subjected to rapid drought stress. Measurements were performed with an infrared gas analyzer at three different times: 50 DAT (before imposing the drought stress), 57 DAT (after 7 days without watering) and 74 DAT (recovery period, 14 days after the resumption of normal watering). The intrinsic water use efficiency (WUE) was calculated by dividing net photosynthesis (P_n) and transpiration rate (E). Data shown are the means, n = 10, error bars indicate SEM. Different letters indicate significant differences in a two-way ANOVA (P-value < 0.05) for each parameter. Uppercase letters indicate the irrigation factor (before drought, during drought or recovery), while lowercase letters indicate the inoculation factor (non-inoculated or N33 + M25). The interaction between irrigation and inoculation factors was no significant in all cases.