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. 2020 Oct 26;11:5405. doi: 10.1038/s41467-020-19259-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a SEC analysis of BBRF2Δ-BSRF1Δ interface mutants (Superdex 75). mAU milli-absorption units. The elution peaks are shown as dashed lines of BBRF2Δ-BSRF1Δ heterodimer (orange), BBRF2Δ monomer (green), BSRF1Δ monomer (purple) are overlaid with each sample (red) as references. b The binding affinity of BBRF2Δ (wild-type or mutant) with BSRF1Δ (wild-type or mutant) measured by BLI. BSRF1Δ (wild-type or mutant) at different concentrations was exposed to 10 μg ml⁻¹ His-tagged BBRF2Δ (wild-type or mutant). Source data are provided as a Source Data file. c Schematic showing the designation of the five BSRF1Δ-derived peptides. N denotes the N-loop. d Binding affinity of peptide 1 (P1) and BBRF2Δ measured by BLI. P1 at different concentrations was exposed to 10 μg ml⁻¹ His-tagged BBRF2Δ. Source data are provided as a Source Data file. e P1 competes with BSRF1Δ in binding to BBRF2Δ. 200 nM BBRF2Δ mixed with different concentrations of P1 was exposed to 10 μg ml⁻¹ immobilized BSRF1Δ. Source data are provided as a Source Data file. f TAT-P1 reduces the numbers of EBV genome copies in a concentration-dependent manner. Error bar indicates s.d. (n = 3). Source data are provided as a Source Data file.