Table 1.

Kinetic constants K_d, k_a, and k_d of the wild-type and mutant GPs.

Mutations	Kd (nM)	ka (M−1 s−1)	kd (s−1)
Wild-type	0.07 ± 0.01	6.46 × 105	4.53 × 10−5
G238A	0.58 ± 0.23	2.88 × 105	16.6 × 10−5
S239A	2.74 ± 0.94	0.30 × 105	8.29 × 10−5
C240A	No binding	No binding	No binding
S241A	0.06 ± 0.03	5.50 × 105	2.99 × 10−5
R242A	0.15 ± 0.002	2.95 × 105	4.52 × 10−5
L305A	0.22 ± 0.08	3.98 × 105	8.17 × 10−5
F458A	0.67 ± 0.09	1.77 × 105	11.70 × 10−5
P488A	0.14 ± 0.06	3.33 × 105	4.64 × 10−5
G489A	1.37 ± 0.14	3.96 × 105	53.5 × 10−5
Q490A	0.07 ± 0.005	5.72 × 105	3.92 × 10−5
W504A	2.09 ± 0.05	4.16 × 105	87.2 × 10−5
E505A	< 0.001	2.00 × 105	< 1.0 × 10−7
G506A	No binding	No binding	No binding
V507A	0.02 ± 0.002	3.63 × 105	0.70 × 10−5
T531A	< 0.001	3.08 × 105	< 1.0 × 10−7
A532W	No binding	No binding	No binding
E533A	< 0.001	1.62 × 105	< 1.0 × 10−7
E554A	< 0.001	2.49 × 105	< 1.0 × 10−7
D555A	0.09 ± 0.002	3.32 × 105	2.85 × 10−5
N557A	No binding	No binding	No binding
A558W	No binding	No binding	No binding
Q559A	< 0.001	6.37 × 105	< 1.0 × 10−7
E579A	< 0.001	6.00 × 105	< 1.0 × 10−7
Y581A	< 0.001	1.46 × 105	< 1.0 × 10−7
T583A	0.06 ± 0.01	5.05 × 105	3.08 × 10−5
D585A	0.05 ± 0.01	3.39 × 105	1.85 × 10−5
N586A	0.30 ± 0.07	2.50 × 105	7.44 × 10−5
I588A	0.36 ± 0.13	1.36 × 105	5.06 × 10−5